Cat Interferon Alpha ELISA Kit with High Spesificity and Sensitivity
Cat.No E0042Cat
Intended Use
This sandwich kit is for the accurate quantitative detection of Cat Interferon Alpha (also known as IFN-A) in serum, plasma, cell culture supernates, cell lysates, tissue homogenates.
Assay Principle
This kit is an Enzyme-Linked Immunosorbent Assay (ELISA). The plate has been pre-coated with Cat IFN-A antibody. IFN-A present in the sample is added and binds to antibodies coated on the wells. And then biotinylated Cat IFN-A Antibody is added and binds to IFN-A in the sample. Then Streptavidin-HRP is added and binds to the Biotinylated IFN-A antibody. After incubation unbound Streptavidin-HRP is washed away during a washing step. Substrate solution is then added and color develops in proportion to the amount of Cat IFN-A. The reaction is terminated by addition of acidic stop solution and absorbance is measured at 450 nm.
Reagent Provided
Components |
Quantity |
Standard Solution (480ng/L) |
0.5ml x1 |
Pre-coated ELISA Plate |
12 * 8 well strips x1 |
Standard Diluent |
3ml x1 |
Streptavidin-HRP |
6ml x1 |
Stop Solution |
6ml x1 |
Substrate Solution A |
6ml x1 |
Substrate Solution B |
6ml x1 |
Wash Buffer Concentrate (30x) |
20ml x1 |
Biotinylated Cat IFN-A Antibody |
1ml x1 |
User Instruction |
1 |
Plate Sealer |
2 pics |
Zipper bag |
1 pic |
Specimen Collection
Serum Allow serum to clot for 10-20 minutes at room temperature. Centrifuge at 2000-3000 RPM for 20 minutes.
Plasma Collect plasma using EDTA or heparin as an anticoagulant. Centrifuge samples for 15 minutes at 2000-3000 RPM at 2 - 8°C within 30 minutes of collection.